MICRO-QUANTITATION OF LENS MYOINOSITOL BY ANION-EXCHANGE CHROMATOGRAPHY

被引：7

作者：

DICKERSON, JE

LOU, MF

机构：

[1] Cataract Research, Alcon Laboratories, Inc., Fort Worth, TX, 76134

来源：

CURRENT EYE RESEARCH | 1990年 / 9卷 / 02期

关键词：

D O I：

10.3109/02713689008995207

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

A method capable of the determination of pmole quantities of myo-inositol in mgr amounts of tissue by anion exchange chromatography is detailed for use in lens and potentially other tissues. Samples were rendered protein-free through ZnS04/Ba(0H)2 precipitation, lyophilized and reconstituted in water just prior to analysis. An aliquot of sample was injected onto an anion exchange column and eluted with a 0.045 M sodium hydroxide mobile phase. Each analysis requires 30 minutes to complete. Average recovery of myo-inositol added to lens sample prior to injection was 100.6% The coefficient of variation for repeated sample injections was 2.9% Rabbit lens averaged 11.4 μmol/gr wet weight with epithelium containing 8.5 μmol/gr wet weight while human lens contained 20.1 μmol/gr wet weight and human epithelial cells had 17.5 nmol/mgr protein. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：201 / 205

页数：5

共 21 条

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