DIFFERENCES IN THE INTERACTIONS OF LIVER ALCOHOL DEHYDROGENASES WITH PROBES BINDING INTO THE SUBSTRATE POCKET

被引:14
作者
KOVAR, J
DURROVA, E
SKURSKY, L
机构
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 101卷 / 02期
关键词
D O I
10.1111/j.1432-1033.1979.tb19745.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The interactions of three groups of probes (berberine alkaloids, tricyclic psychopharmaca and acridine derivatives) with isoenzymes of horse liver alcohol dehydrogenase and with rat liver alcohol dehydrogenase have been examined. These compounds inhibit the activity of the EE isoenzyme of horse liver alcohol dehydrogenase but differ in their behaviour towards the steroid‐active enzymes (i.e. the ES isoenzyme of horse liver alcohol dehydrogenase and alcohol dehydrogenase from rat liver): psychopharmaca inhibit, acridines activate and berberines do not bind. The ligands differ also in their influence on the modification of the EE isoenzyme by iodoacetate. Polarities (expressed as Kosower's Z values) of the respective binding sites on the EE isoenzyme were estimated from optical properties of bound probes. Berberines bind into a very hydrophobic area of the enzyme molecule, the binding site for psychopharmaca is moderately hydrophobic and that for acridines is rather polar. Steric arrangements of the binding sites are also discussed. The data presented confirm the existence of three distinct binding sites for these ligands in the substrate pocket of liver alcohol dehydrogenase. Copyright © 1979, Wiley Blackwell. All rights reserved
引用
收藏
页码:507 / 514
页数:8
相关论文
共 23 条
  • [1] Branden C-I., 1975, ENZYMES, V11, P103, DOI 10.1016/S1874-6047(08)60211-5
  • [2] DURROVA E, 1978, THESIS U BRNO
  • [3] CATALYSIS OF PHOTOCHEMICAL DISMUTATION OF N-METHYLACRIDINIUM CATION TO N-METHYLACRIDONE AND N-METHYL-9, 10-DIHYDROACRIDINE BY HYDROPHOBIC SITES AND HUMAN-SERUM ALBUMIN
    EINARSSON, R
    ZEPPEZAUER, M
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 59 (01): : 295 - 304
  • [4] STRUCTURAL COMPARISONS OF MAMMALIAN, YEAST AND BACILLAR ALCOHOL DEHYDROGENASES
    EKLUND, H
    BRANDEN, CI
    JORNVALL, H
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1976, 102 (01) : 61 - &
  • [5] 3-DIMENSIONAL STRUCTURE OF HORSE LIVER ALCOHOL-DEHYDROGENASE AT 2.4 A RESOLUTION
    EKLUND, H
    NORDSTROM, B
    ZEPPEZAUER, E
    SODERLUND, G
    OHLSSON, I
    BOIWE, T
    SODERBERG, BO
    TAPIA, O
    BRANDEN, CI
    AKESON, A
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1976, 102 (01) : 27 - &
  • [6] FRANKE R, 1976, QUANTITATIVE STRUCTU, P25
  • [7] HEITZ JR, 1971, BIOCHEMISTRY-US, V10, P2695
  • [8] KOGLIN W, 1952, KURZES HDB CHEM, V2, P695
  • [10] FLUORESCENCE STUDY OF LIVER-ALCOHOL-DEHYDROGENASE COMPLEXES WITH BERBERINE AND OTHER LIGNDS
    KOVAR, J
    SKURSKY, L
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1973, 40 (01): : 233 - 244