AN AUTOMATED SPECTROPHOTOMETRIC FLOW-INJECTION PROCEDURE FOR THE DETERMINATION OF CELLULASE ACTIVITY IN BIOREACTOR PREPARATIONS

被引：12

作者：

WORSFOLD, PJ ^{[1
]}

WHITESIDE, IRC ^{[1
]}

PFEIFFER, HF ^{[1
]}

WALDHOFF, H ^{[1
]}

机构：

[1] HENKEL KGAA,RES & DEV,DUSSELDORF,GERMANY

来源：

JOURNAL OF BIOTECHNOLOGY | 1990年 / 14卷 / 01期

关键词：

Cellulase activity; Flow-injection; p-Aminobenzoylhydrazide;

D O I：

10.1016/0168-1656(90)90020-C

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A spectrophotometric procedure for the determination of cellulase activity in precipitated bioreactor preparations and culture filtrates is described. It is based on the determination of reducing sugar produced by the action of the enzyme on carboxymethylcellulose. The reducing sugar is derivatized with p-aminobenzoylhydrazide and permits a limit of detection of 0.1 U ml-1 cellulase in the presence of background sugar, with a sampling rate of 5 h-1. The method can readily be applied to the determination of any carbohydrase acting on soluble substrates and producing reducing sugars, e.g. amylase, dextranase, xylanase, glucanase and polygalacturonase. © 1990.

引用

页码：81 / 87

页数：7

共 10 条

[1] CONTINUOUS ONLINE MONITORING OF INTRACELLULAR ENZYME-ACTIVITY [J].