OPTIMIZATION OF CONDITIONS FOR THE SIMULTANEOUS SEPARATION OF 10 TRYPTOPHAN-METABOLITES USING REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：8

作者：

CHUANG, CZ

RAGAN, FA

PRASAD, C

机构：

[1] PENNINGTON BIOMED RES CTR, NEUROSCI LAB, 6400 PERKINS RD, BATON ROUGE, LA 70808 USA

[2] LOUISIANA STATE UNIV, MED CTR, DEPT PATHOL, NEW ORLEANS, LA 70112 USA

[3] DEPT MED, ENDOCRINOL SECT, NEW ORLEANS, LA 70112 USA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 534卷

关键词：

D O I：

10.1016/S0378-4347(00)82144-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic (RP-HPLC) method for the separation of tryptophan and ten metabolites of tryptophan pyrrolase pathway has been developed by sequential optimization of mobile phase, by adjusting the pH, the concentration of triethylamine and the gradient elution. The baseline resolution of the compounds, by this optimized procedure, is obtained with an analysis time, including the re-equilibration period, of less than 30 min. We believe this is the first RP-HPLC method that can separate tryptophan and ten of its metabolites in a single chromatographic run.

引用

收藏

页码：13 / 21

页数：9

相关论文

共 33 条

[1] SEPARATION OF IONIC COMPOUNDS BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY - AN UPDATE OF ION-PAIRING TECHNIQUES [J].

BIDLINGMEYER, BA .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1980, 18 (10) :525-539

[2] SIMULTANEOUS-OPTIMIZATION OF REAGENT CONCENTRATION AND PH IN REVERSED-PHASE ION-PAIRING CHROMATOGRAPHY [J].

BILLIET, HAH ;

VUIK, J ;

STRASTERS, JK ;

DEGALAN, L .

JOURNAL OF CHROMATOGRAPHY, 1987, 384 :153-162

[3] APPLICATION OF A MIXTURE DESIGN TECHNIQUE TO THE SIMULTANEOUS-OPTIMIZATION OF ANALYSIS TIME AND RESOLUTION IN REVERSED-PHASE ION-PAIR LIQUID-CHROMATOGRAPHY USING A MINIMAL RESOLUTION PLOT [J].

COENEGRACHT, PMJ ;

VANTUYEN, N ;

METTING, HJ ;

COENEGRACHTLAMERS, PJM .

JOURNAL OF CHROMATOGRAPHY, 1987, 389 (02) :351-367

[4] INCREASE IN KYNURENIC ACID IN HUNTINGTONS-DISEASE MOTOR CORTEX [J].

CONNICK, JH ;

CARLA, V ;

MORONI, F ;

STONE, TW .

JOURNAL OF NEUROCHEMISTRY, 1989, 52 (03) :985-987

[5] GRADIENT ELUTION IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY .2. PRACTICAL APPLICATION TO REVERSED-PHASE SYSTEMS [J].

DOLAN, JW ;

GANT, JR ;

SNYDER, LR .

JOURNAL OF CHROMATOGRAPHY, 1979, 165 (01) :31-58

[6] THE NEUROTOXIC ACTIONS OF QUINOLINIC ACID IN THE CENTRAL-NERVOUS-SYSTEM [J].

ELDEFRAWY, SR ;

BOEGMAN, RJ ;

JHAMANDAS, K ;

BENINGER, RJ .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1986, 64 (03) :369-375

[7] SEPARATION OF NUCLEOTIDES BY ION-PAIR, REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - USE OF MG(II) AND TRIETHYLAMINE AS COMPETING HETAERONS IN THE SEPARATION OF ADENINE AND GUANINE-NUCLEOTIDES [J].

FOLLEY, LS ;

POWER, SD ;

POYTON, RO .

JOURNAL OF CHROMATOGRAPHY, 1983, 281 (DEC) :199-207

[8] KYNURENINE METABOLITES OF TRYPTOPHAN - IMPLICATIONS FOR NEUROLOGIC DISEASES [J].

FREESE, A ;

SWARTZ, KJ ;

DURING, MJ ;

MARTIN, JB .

NEUROLOGY, 1990, 40 (04) :691-695

[9] SYNTHESIS AND METABOLISM OF L-KYNURENINE IN RAT-BRAIN [J].

GAL, EM ;

SHERMAN, AD .

JOURNAL OF NEUROCHEMISTRY, 1978, 30 (03) :607-613

[10] PRACTICAL ASPECTS OF REVERSE PHASE ION-PAIR CHROMATOGRAPHY [J].

GLOOR, R ;

JOHNSON, EL .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1977, 15 (09) :413-423

← 1 2 3 4 →