TRANSLATION ENHANCING PROPERTIES OF THE 5'-LEADER OF POTATO VIRUS-X GENOMIC RNA

被引：26

作者：

ZELENINA, DA ^{[1
]}

KULAEVA, OI ^{[1
]}

SMIRNYAGINA, EV ^{[1
]}

SOLOVYEV, AG ^{[1
]}

MIROSHNICHENKO, NA ^{[1
]}

FEDORKIN, ON ^{[1
]}

RODIONOVA, NP ^{[1
]}

MOROZOV, SY ^{[1
]}

ATABEKOV, JG ^{[1
]}

机构：

[1] MV LOMONOSOV STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR

来源：

FEBS LETTERS | 1992年 / 296卷 / 03期

关键词：

RECOMBINANT DNA; TRANSLATIONAL ENHANCEMENT; PLANT VIRUS;

D O I：

10.1016/0014-5793(92)80301-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The double-stranded DNA copy corresponding to the 5'-nontranslated alpha-beta-leader of potato virus X (PVX) genomic RNA (positions -3 to -85 according to AUG initiator) was chemically synthesized and fused to the transcription plasmids containing three different reporter genes: neomycinphosphotransferase type II (NPT II) gene, Bacillus thuringiensis coleopteran-specific toxic protein gene and beta-glucuronidase (GUS) gene. Expression of the reporter genes in vitro and in plant protoplasts (in the case of GUS gene) reveals that the alpha-beta-leader of PVX RNA acts as a translation enhancer despite the presence of the upstream vector-derived sequence and irrespective of the length of the spacer sequence preceding the reporter genes.

引用

页码：267 / 270

页数：4

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