CHARACTERIZATION OF THE PRECURSOR FOR MANDUCA-SEXTA DIURETIC HORMONE MAS-DH

被引：26

作者：

DIGAN, ME ^{[1
]}

ROBERTS, DN ^{[1
]}

ENDERLIN, FE ^{[1
]}

WOODWORTH, AR ^{[1
]}

KRAMER, SJ ^{[1
]}

机构：

[1] SANDOZ AGRO INC,PALO ALTO,CA 94304

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 22期

关键词：

WATER BALANCE; ENDOPROTEOLYTIC CLEAVAGE; SECRETION; TISSUE-SPECIFIC REGULATION;

D O I：

10.1073/pnas.89.22.11074

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have isolated a cDNA clone encoding a precursor form of the diuretic hormone from the tobacco hornworm Manduca sexta (Mas-DH). Translation of the cDNA revealed a 138-amino acid precursor consisting of the Mas-DH amino acid sequence bounded by dibasic amino acid processing sites, a putative signal sequence, and additional peptide sequence on either side of the Mas-DH coding sequence. The region of the precursor upstream of the mature Mas-DH sequence shows limited (28%) homology to the cryptic region of the ovine corticotropin-releasing factor precursor. The Mas-DH RNA is 1.5-1.6 kilobases long; it is present in both the heads and bodies of adult and larval insects. In prewandering fifth stadium larvae, Mas-DH mRNA is expressed in brain, nerve cord, gut, and Malpighian tubules, but not in the fat body. There is a single genomic copy of the Mas-DH gene; the message is multiply spliced.

引用

页码：11074 / 11078

页数：5

共 30 条

[1] INVIVO FLUCTUATION OF JH, JH ACID, AND ECDYSTEROID TITER, AND JH ESTERASE-ACTIVITY, DURING DEVELOPMENT OF 5TH STADIUM MANDUCA-SEXTA [J].