PROLACTIN INDUCES GROWTH-INHIBITION AND PROMOTES DIFFERENTIATION OF CHO CELLS STABLY TRANSFECTED WITH PROLACTIN RECEPTOR COMPLEMENTARY-DNA

被引：7

作者：

BIGNON, C ^{[1
]}

DANIEL, N ^{[1
]}

KERMABON, AY ^{[1
]}

DJIANE, J ^{[1
]}

机构：

[1] INRA,UNITE ENDOCRINOL MOLEC,F-78352 JOUY EN JOSAS,FRANCE

来源：

FEBS LETTERS | 1995年 / 358卷 / 01期

关键词：

PROLACTIN RECEPTOR; GROWTH INHIBITION; DIFFERENTIATION MARKER; SIGNAL TRANSDUCTION; TRANSFECTION;

D O I：

10.1016/0014-5793(94)01400-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have characterized a stable and functional transfectant of the rabbit prolactin receptor in Chinese hamster ovary cells, and investigated the action of prolactin (PRL) on the growth and differentiation of this transfectant (clone E32). PRL induced a significant inhibition of E32 cell proliferation. Growth inhibition correlated with gene induction of the molecular marker of ovarian differentiation cholesterol side chain cleavage P450 (P450(scc)). Both effects were inversely proportional to cell confluence. The limits and potential development of such transfected cellular systems are discussed.

引用

页码：84 / 88

页数：5

共 30 条

[1]

ARGETSINGER LS, 1993, CELL, V74, P1

[2]

BIGNON C, 1993, BIOTECHNIQUES, V15, P243

[3]

CAHOREAU C, 1992, BIOCHIMIE

[4] GROWTH-HORMONE AND ADIPOSE DIFFERENTIATION - GROWTH HORMONE-INDUCED ANTIMITOGENIC STATE IN 3T3-F442A PREADIPOSE CELLS [J].

CORIN, RE ;

GULLER, S ;

WU, KY ;

SONENBERG, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (19) :7507-7511

[5] EVOLUTION OF PROLACTIN RECEPTORS IN RABBIT MAMMARY-GLAND DURING PREGNANCY AND LACTATION [J].

DJIANE, J ;

DURAND, P ;

KELLY, PA .

ENDOCRINOLOGY, 1977, 100 (05) :1348-1356

[6]

DJIANE J, 1985, J BIOL CHEM, V260, P1430

[7] LACTOGENIC HORMONE AND CELL TYPE-SPECIFIC CONTROL OF THE WHEY ACIDIC PROTEIN GENE PROMOTER IN TRANSFECTED MOUSE CELLS [J].

DOPPLER, W ;

VILLUNGER, A ;

JENNEWEIN, P ;

BRDUSCHA, K ;

GRONER, B ;

BALL, RK .

MOLECULAR ENDOCRINOLOGY, 1991, 5 (11) :1624-1632

[8] PROLACTIN RECEPTOR GENE-EXPRESSION IN THE RABBIT - IDENTIFICATION, CHARACTERIZATION AND TISSUE DISTRIBUTION OF SEVERAL PROLACTIN RECEPTOR MESSENGER-RNAS ENCODING A UNIQUE PRECURSOR [J].

DUSANTERFOURT, I ;

GAYE, P ;

BELAIR, L ;

PETRIDOU, B ;

KELLY, PA ;

DJIANE, J .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1991, 77 (1-3) :181-192

[9] IDENTIFICATION OF JAK PROTEIN-TYROSINE KINASES AS SIGNALING MOLECULES FOR PROLACTIN - FUNCTIONAL-ANALYSIS OF PROLACTIN RECEPTOR AND PROLACTIN ERYTHROPOIETIN RECEPTOR CHIMERA EXPRESSED IN LYMPHOID-CELLS [J].

DUSANTERFOURT, I ;

MULLER, O ;

ZIEMIECKI, A ;

MAYEUX, P ;

DRUCKER, B ;

DJIANE, J ;

WILKS, A ;

HARPUR, AG ;

FISCHER, S ;

GISSELBRECHT, S .

EMBO JOURNAL, 1994, 13 (11) :2583-2591

[10] IMMUNOLOGICAL RECOGNITION OF THE PROLACTIN RECEPTOR - IDENTIFICATION OF A SINGLE BINDING UNIT OF MOLECULAR-WEIGHT - 42000 [J].

DUSANTERFOURT, I ;

KELLY, PA ;

DJIANE, J .

BIOCHIMIE, 1987, 69 (6-7) :639-646

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