PENICILLIUM URTICAE BAINIER ENUMERATION IN SOILS

A dilution plating method estimated Penicillium urticae Bainier numbers in soil. This method, which used an agar layering technique and a cyclic incubation of 8 hours at room temperature (about 25°C) and 16 hours at 5°C, permitted the differential growth and sporulation in favor of P. urticae B. over other common soil fungi. Procedures of extraction, paper chromatography, infrared analysis, and bioassay assayed for accumulated patulin. A combination of these methods routinely estimated P. urticae B. numbers in soils and authenticated patulin production by selected isolates. © 1969 Martinus Nijhoff.