BIOSYNTHESIS OF MSH AND RELATED PEPTIDES IN THE PARS-INTERMEDIA OF THE MOUSE - PULSE-CHASE ANALYSIS

被引：8

作者：

JENKS, BG

MEULEPAS, WJAM

SOONS, PJA

VANOVERBEEKE, AP

机构：

[1] Department of Zoology, University of Nijmegen, Toernooiveld

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1979年 / 13卷 / 02期

关键词：

ACTH; biosynthesis; MSH; pars intermedia; precursor; pulse-chase;

D O I：

10.1016/0303-7207(79)90015-7

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Protein biosynthesis in neurointermediate lobes of mouse pituitaries was investigated using pulse and pulse-chase techniques with [3H]lysine. Electrophoretic analysis of lobe homogenates on acid-urea gels resolved 11 labeled products. One was a large protein which was rapidly synthesized during pulse-incubations and disappeared during chase incubations. Three of the products increased during chase incubations, suggesting a precursor-product mode of biosynthesis for these peptides. One of these three products co-migrated with synthetic α-MSH and also corresponds to the major peak of mouse neurointermediate lobe MSH bioactivity and immunoactivity on electrophoretograms. Another case of these chase peptides has electrophoretic properties similar to those of ACTH. © 1979.

引用

页码：149 / 158

页数：10

共 19 条

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Baker B I, 1977, Front Horm Res, V4, P66

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