TRANSMEMBRANE SIGNALING BY THE N-FORMYL PEPTIDE RECEPTOR IN STABLY TRANSFECTED FIBROBLASTS

被引：52

作者：

PROSSNITZ, ER ^{[1
]}

QUEHENBERGER, O ^{[1
]}

COCHRANE, CG ^{[1
]}

YE, RD ^{[1
]}

机构：

[1] Scripps Res Inst, RES INST, DEPT IMMUNOL, LA JOLLA, CA 92037 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)91394-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the requirement for N-formyl peptide receptor-mediated transmembrane signalling in transfected mouse fibroblasts that express the receptor. Stably transfected cells displayed specific binding for N-formyl-Met-Leu-[3H]Phe with a dissociation constant of 3 nM. The cells responded to ligand stimulation with mobilization of calcium from intracellular stores. Calcium mobilization was ligand dose-dependent (EC50=3 nM fMet-Leu-Phe) and could be inhibited by pertussis toxin treatment. These results provide the first demonstration that expression of the single-chain N-formyl peptide receptor in mouse fibroblasts is sufficient for mediating ligand-induced early transmembrane signalling events, which do not appear to require other neutrophil-specific cellular components. © 1991.

引用

页码：471 / 476

页数：6

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