CHEMICAL NATURE OF INVIVO DNA-BASE DAMAGE IN HYDROGEN PEROXIDE-TREATED MAMMALIAN-CELLS

被引：112

作者：

DIZDAROGLU, M

NACKERDIEN, Z

CHAO, BC

GAJEWSKI, E

RAO, G

机构：

[1] NATL INST STAND & TECHNOL,CTR CHEM TECHNOL,GAITHERSBURG,MD 20899

[2] UNIV STELLENBOSCH,DEPT RADIOTHERAPY,TYGERBERG 7505,SOUTH AFRICA

[3] UNIV MARYLAND,CHEM & BIOCHEM ENGN PROGRAM,CATONSVILLE,MD 21228

[4] UNIV MARYLAND,CTR MED BIOTECHNOL,MARYLAND BIOTECHNOL INST,CATONSVILLE,MD 21228

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1991年 / 285卷 / 02期

基金：

美国国家科学基金会; 英国医学研究理事会;

关键词：

D O I：

10.1016/0003-9861(91)90378-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrogen peroxide is generated in mammalian cells by normal metabolism or by treatment with external agents. Treatment of mammalian cells with this oxidizing agent results in DNA damage. Little is known about the chemical nature of hydrogen peroxide-mediated DNA damage in mammalian cells. Here we report on the chemical characterization of in vivo base damage to nuclear DNA in mammalian cells caused by exposure to H2O2. Chromatin was isolated from cells and analyzed by gas chromatography/mass spectrometry with selected-ion monitoring. Ten DNA base products were identified and quantitated. Modified bases identified were typical hydroxyl radical-induced products of DNA bases. Results indicate involvement of hydroxyl radicals in the mechanism of nuclear DNA damage in mammalian cells caused by H2O2. © 1991.

引用

页码：388 / 390

页数：3

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