PHOTOKINETIC MICRO-ASSAY OF ADENYLATE KINASE USING THE FIREFLY LUCIFERASE REACTION

被引：6

作者：

BROLIN, SE

BORGLUND, E

AGREN, A

机构：

[1] Department of Histology, Biomedicum, University of Uppsala, Uppsala

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 03期

关键词：

adenylate kinase; bioenergetics; bioluminescene analysis; firefly luciferase; photokinetics;

D O I：

10.1016/0165-022X(79)90035-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new rapid photokinetic method is described for determining the activity of adenylate kinase (ATP:AMP phosphotranspherase, EC 2.7.4.3) in 0.1-5.0 μg of freeze-dried tissue. This represents a weight range far below that obtainable by fine-needle biopsy. The reaction 2 ADP ⇌ ATP was employed and the ATP formed assayed with firefly luciferase as light yielder. The light emission was recorded on a multi-channel scaler. The adenylate kinase activities found in tissues of mice were in the same range as previously described in a study using fluorometric microassay. © 1979.

引用

页码：163 / 169

页数：7

共 12 条

[1] GLUCOSE-METABOLISM OF PANCREATIC-ISLETS ISOLATED FROM NEONATAL RATS [J].