EVIDENCE FOR THE EXISTENCE OF CYTOSKELETON-BOUND POLYSOMES IN PLANTS

被引：51

作者：

DAVIES, E ^{[1
]}

FILLINGHAM, BD ^{[1
]}

OTO, Y ^{[1
]}

ABE, S ^{[1
]}

机构：

[1] EHIME UNIV,COLL AGR,MATSUYAMA,EHIME 790,JAPAN

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1991年 / 15卷 / 10期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1016/0309-1651(91)90147-B

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

When conventional, high ionic strength buffers were used for the isolation of polysomes from pea plante, less than 20% were retained in the detergent-insoluble pellet. Reducing Tris, K+ and Mg++ to 10 mM increased retention to 70%, and when a new, microfilament-stabilizing buffer was used, retention increased to 80%. Conditions which favoured polysome pelleting at lower g forces permitted the retention of actin in the pellet. The data are consistent with the hypothesis that higher plants, like animals, contain cytoskeleton-(actin)-bound polysomes. © 1991.

引用

页码：973 / 981

页数：9

共 16 条

[1]

ABE S, 1985, PLANT CELL PHYSIOL, V26, P1499

[2]

ABE S, 1991, IN PRESS PROTOPLASMA

[3] POLYRIBOSOME DEGRADATION AS A SENSITIVE ASSAY FOR ENDOLYTIC MESSENGER-RIBONUCLEASE ACTIVITY [J].