THE USE OF BIDIRECTIONAL TRANSCRIPTION FOOTPRINTING TO DETECT PLATINUM-DNA CROSS-LINKS BY ACRIDINE-TETHERED PLATINUM DIAMINE COMPLEXES AND CISPLATIN

被引:35
作者
CULLINANE, C
WICKHAM, G
MCFADYEN, WD
DENNY, WA
PALMER, BD
PHILLIPS, DR
机构
[1] LA TROBE UNIV,DEPT BIOCHEM,BUNDOORA,VIC 3083,AUSTRALIA
[2] VICTORIAN COLL PHARM,SCH PHARMACEUT CHEM,PARKVILLE,VIC 3052,AUSTRALIA
[3] UNIV MELBOURNE,INST EDUC,SCH SCI & MATHS EDUC,DIV CHEM & PHYS,CARLTON,VIC 3053,AUSTRALIA
[4] UNIV AUCKLAND,SCH MED,CANC RES LAB,AUCKLAND,NEW ZEALAND
基金
澳大利亚研究理事会;
关键词
D O I
10.1093/nar/21.3.393
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bidirectional transcription footprinting has been used to probe the platination of DNA by cisplatin, and to examine the modulation of these interactions by (a) cyclisation of the non-reactive amino group by either ethyl or propyl groups, and (b) the further addition of a pendant intercalator (9-amino acridine) linked by either phenylethyl or phenylpentyl groups. Intrastrand crosslinking was detected for all derivatives at all 5'-GG and 5'-AG sequences on the template strand, but the same sites did not result in transcriptional blockages when on the non-template strand. There was little effect of cyclysation of the amino groups, but the further addition of an intercalator resulted in three responses: a time-dependent increase of the blocked transcript by one and three nucleotides; a reduction of the sequence selectivity of platination; a decrease of apparent interstrand crosslinking for these derivatives with a pendant intercalator tethered to the amino moiety of cisplatin.
引用
收藏
页码:393 / 400
页数:8
相关论文
共 21 条
[1]   DNA UNWINDING PRODUCED BY SITE-SPECIFIC INTRASTRAND CROSS-LINKS OF THE ANTITUMOR DRUG CIS-DIAMMINEDICHLOROPLATINUM(II) [J].
BELLON, SF ;
COLEMAN, JH ;
LIPPARD, SJ .
BIOCHEMISTRY, 1991, 30 (32) :8026-8035
[2]   MODULATION OF PLATINUM ANTITUMOR DRUG-BINDING TO DNA BY LINKED AND FREE INTERCALATORS [J].
BOWLER, BE ;
LIPPARD, SJ .
BIOCHEMISTRY, 1986, 25 (10) :3031-3038
[3]  
BRUHN SJ, 1990, BIOINORG CHEM, V38, P477
[4]   RNA-POLYMERASES REACT DIFFERENTLY AT D(APG) AND D(GPG) ADDUCTS IN DNA MODIFIED BY CIS-DIAMMINEDICHLOROPLATINUM(II) [J].
CORDA, Y ;
ANIN, MF ;
LENG, M ;
JOB, D .
BIOCHEMISTRY, 1992, 31 (07) :1904-1908
[5]   INDUCTION OF STABLE TRANSCRIPTIONAL BLOCKAGE SITES BY ADRIAMYCIN - GPC SPECIFICITY OF APPARENT ADRIAMYCIN DNA ADDUCTS AND DEPENDENCE ON IRON(III) IONS [J].
CULLINANE, C ;
PHILLIPS, DR .
BIOCHEMISTRY, 1990, 29 (23) :5638-5646
[6]   INVITRO TRANSCRIPTION ANALYSIS OF DNA ADDUCTS INDUCED BY CYANOMORPHOLINOADRIAMYCIN [J].
CULLINANE, C ;
PHILLIPS, DR .
BIOCHEMISTRY, 1992, 31 (40) :9513-9519
[8]   INVITRO TRANSCRIPTION ANALYSIS OF DNA ALKYLATION BY NITROGEN-MUSTARD [J].
GRAY, PJ ;
CULLINANE, C ;
PHILLIPS, DR .
BIOCHEMISTRY, 1991, 30 (32) :8036-8040
[9]  
JOHNSON GL, 1966, INORG SYNTH, V8, P242
[10]   CIS- AND TRANS-DICHLORODIAMMINEPLATINUM(II) [J].
KAUFFMAN, GB ;
COWAN, DO .
INORGANIC SYNTHESES, 1963, 7 :239-245