COEXPRESSION OF EPSTEIN-BARR-VIRUS LATENT MEMBRANE-PROTEIN AND VIMENTIN IN AGGRESSIVE HISTOLOGICAL SUBTYPES OF HODGKINS-DISEASE

被引:19
作者
CARBONE, A
GLOGHINI, A
ZANETTE, I
CANAL, B
RIZZO, A
VOLPE, R
机构
[1] Division of Pathology, Centro di Riferimento Oncologico, Istituto Nazionale di Ricovero e Cura a Carattere Scientifico, Aviano, I-33081, Via Pedemontana Occidentale
关键词
HODGKINS DISEASE; EPSTEIN-BARR VIRUS; LATENT MEMBRANE PROTEIN; VIMENTIN; MESSENGER RNA;
D O I
10.1007/BF01605131
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The presence of Epstein-Barr virus (EBV) genome in Hodgkin's and Reed-Sternberg (HRS) cells, as detected using in situ hybridization (ISH) with biotinylated BamHI ''V'' probes, along with the expression of EBV-encoded latent membrane protein (LMP) and vimentin was examined in paraffin-embedded sections of 39 immunomorphologically characterized cases of Hodgkin's disease (HD). ISH demonstrated EBV in HRS cells in 15 of 39 cases, whereas LMP expression was detected in 11 of 39 cases, only in the presence of EBV genome detection. With the exception of 1 case, in which HRS cells expressed B-cell-associated antigens, the LMP-positive cases included specimens in which HRS cells were of non-B, non-T phenotype. LMP expression showed a stronger association with lymphocyte depletion (LD) (3/3) and mixed cellularity (MC) (6/11) than with lymphocyte predominance (0/5) or nodular sclerosis (2/20) subtypes. Vimentin expression on HRS cells was found in all the LMP-expressing cases and only in a fraction (13/28) of LMP-negative cases. This study supports the view that HD represents a heterogeneous group of diseases also in terms of EBV association, LMP expression being strongly related to the ''aggressive'' LD and MC histological subtypes. In light of the supposed interactions between vimentin and LMP, their co-expression on HRS cells, as detected in this study, provides further evidence for a significant role of EBV in the development of a proportion of HD cases.
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页码:39 / 45
页数:7
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