INTERLEUKIN-6 PRODUCTION DURING CHRONIC EXPERIMENTAL-INFECTION

The appearance of interleukin-6 (IL-6) in serum of mice was monitored during the course of chronic infection with either Brucella abortus vaccine strain 19 or a virulent Mycobacterium avium Complex (MAC) isolate. Serum IL-6 during brucella infection was higher than during infection with MAC, despite similar numbers of bacteria. Furthermore, IL-6 titres decreased after the peak of infection, falling to baseline levels before these chronic infections were eradicated. The ability of peritoneal cells or spleen cell suspensions to produce IL-6 under either specific or non-specific stimulus was greatly enhanced by infection. While production of IL-6 by these cultures was apparently mostly independent of T cells, T cells from infected mice could produce an IL-6 response. Thus CD4+ T lymphocytes prepared from mice which had recovered from B. abortus infection, cultured with antigen and antigen presenting cells, resulted in IL-6 production, which was not observed in similarly cultured CD8+ T cells, indicating a role for T cells.