SOME EXAMPLES OF THE USE OF THIN-LAYER SPECTROELECTROCHEMISTRY IN THE STUDY OF ELECTRON-TRANSFER BETWEEN METALS AND ENZYMES

Some examples of the use of optically transparent thin layer electrodes to study electron transfer between platinum or carbon and enzymes are given. With respect to the biomolecule, thin layer spectroelectrochemistry has been used to choose the best electrochemical pretreatment necessary to promote the heterogeneous electron transfer (in the case of lactate dehydrogenase and glucose oxidase), to visualize intermediates in electrochemical reactions (in the case of glucose oxidase and horseradish peroxidase), to determine the electron transfer rate constant between oxidized, intermediate and reduced forms, and to prepare intermediates compounds (in the case of compound III of horseradish peroxidase), or to regenerate by electrochemical means enzymatic cofactors (in the case of hydrogenase).