A GROUP-I INTRON IN THE MITOCHONDRIAL LARGE-SUBUNIT RIBOSOMAL-RNA-ENCODING GENE OF DICTYOSTELIUM-DISCOIDEUM - SAME SITE LOCALIZATION IN ALGA AND IN-VITRO SELF-SPLICING

被引：14

作者：

ANGATA, K ^{[1
]}

OGAWA, S ^{[1
]}

YANAGISAWA, K ^{[1
]}

TANAKA, Y ^{[1
]}

机构：

[1] UNIV TSUKUBA,INST BIOL SCI,TSUKUBA,IBARAKI 305,JAPAN

来源：

GENE | 1995年 / 153卷 / 01期

关键词：

RIBOZYME; INTRON DISTRIBUTION; CELLULAR SLIME MOLD; POLYSPHONDYLIUM PALLIDUM; DICTYOSTELIUM MUCOROIDES;

D O I：

10.1016/0378-1119(94)00732-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A 547-bp group-I intron belonging to subgroup IA1 was found near the 3' end of the large subunit ribosomal RNA-encoding gene (LSUrRNA) in the mitochondrial (mt) DNA of the cellular slime mold Dictyostelium discoideum. This intron was inserted in a highly conserved stretch within the sequence that encodes the peptidyl transferase center domain V in the corresponding region of the Escherichia coli LSUrRNA. Interestingly, the insertion site of the intron is the same as that of the So.LSU.2 intron of the green alga, Scenedesmus obliquus, mt DNA and the Pw.LSU.2 intron of the colorless alga, Prototheca wickerhamii, mt DNA. The intron could self-splice in vitro at a concentration higher than 20 mM MgCl2. Polymerase chain reaction analysis showed the possible existence of an intron similar to that of D. discoideum LSUrRNA in another cellular slime mold, Polysphondylium pallidum (CK-8), but not in D. mucoroides (Dm7 and Dm11).

引用

页码：49 / 55

页数：7

共 31 条

[1] DISTRIBUTION OF GP138, A CELL-SURFACE PROTEIN RESPONSIBLE FOR SEXUAL CELL-FUSION, AMONG CELLULAR SLIME-MOLDS [J].