PURIFICATION AND CHARACTERIZATION OF A TROPHIC FACTOR FOR EMBRYONIC PERIPHERAL NEURONS - COMPARISON WITH FIBROBLAST GROWTH-FACTORS

被引:115
作者
ECKENSTEIN, FP [1 ]
ESCH, F [1 ]
HOLBERT, T [1 ]
BLACHER, RW [1 ]
NISHI, R [1 ]
机构
[1] ATHENA NEUROSCI,S SAN FRANCISCO,CA 94080
关键词
D O I
10.1016/0896-6273(90)90120-5
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The validation of NGF as a physiologically important neurotrophic factor has led to intense efforts to identify novel polypeptide growth factors for neurons. We report here the details of a >80,000-fold purification of a neurotrophic molecule, referred to as growth-promoting activity (GPA), from chicken sciatic nerves. The final product of the purification migrated as a protein band of 21.5 kd, its apparent pi was approximately 4.8, and the ED50 of the most active preparation was approximately 10 pg/ml. Amino acid sequence of a proteolytic digestion fragment of GPA revealed homology with the recently published sequences for rabbit and rat sciatic nerve CNTF Thus this molecule may be the chicken form of CNTF Analysis of the specificity of action of GPA showed that, in addition to E8 ciliary ganglion neurons, the factor was able to support short-term survival of E8 dorsal root ganglion and E12 sympathetic neurons. This range of specificities of biological action was also seen with both acidic and basic FGF in the presence of heparin. The biological activity of GPA differed from that of FGF in that it was not potentiated by heparin and did not stimulate mitogenesis in chick fibroblasts. © 1990.
引用
收藏
页码:623 / 631
页数:9
相关论文
共 26 条
[1]   PURIFICATION OF THE CHICK EYE CILIARY NEURONOTROPHIC FACTOR [J].
BARBIN, G ;
MANTHORPE, M ;
VARON, S .
JOURNAL OF NEUROCHEMISTRY, 1984, 43 (05) :1468-1478
[2]   WHAT, IF ANYTHING, IS A NEUROTROPHIC FACTOR [J].
BARDE, YA .
TRENDS IN NEUROSCIENCES, 1988, 11 (08) :343-346
[3]  
BERG DK, 1984, ANNU REV NEUROSCI, V7, P149
[4]   THE HEPARIN-BINDING (FIBROBLAST) GROWTH-FACTOR FAMILY OF PROTEINS [J].
BURGESS, WH ;
MACIAG, T .
ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :575-606
[5]   ROLE OF NEUROTROPHIC FACTORS IN DEVELOPMENT [J].
DAVIES, AM .
TRENDS IN GENETICS, 1988, 4 (05) :139-143
[6]   PROLIFERATION AND DIFFERENTIATION OF EMBRYONIC CHICK SYMPATHETIC NEURONS - EFFECTS OF CILIARY NEUROTROPHIC FACTOR [J].
ERNSBERGER, U ;
SENDTNER, M ;
ROHRER, H .
NEURON, 1989, 2 (03) :1275-1284
[7]   CILIARY NEUROTROPHIC FACTOR INDUCES TYPE-2 ASTROCYTE DIFFERENTIATION IN CULTURE [J].
HUGHES, SM ;
LILLIEN, LE ;
RAFF, MC ;
ROHRER, H ;
SENDTNER, M .
NATURE, 1988, 335 (6185) :70-73
[8]   AN ENZYME-RELEASE ASSAY FOR NATURAL CYTO-TOXICITY [J].
KORZENIEWSKI, C ;
CALLEWAERT, DM .
JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 64 (03) :313-320
[9]   SYNAPTIC TRANSMISSION AND CELL-DEATH DURING NORMAL GANGLIONIC DEVELOPMENT [J].
LANDMESSER, L ;
PILAR, G .
JOURNAL OF PHYSIOLOGY-LONDON, 1974, 241 (03) :737-749
[10]   PURIFICATION, CLONING, AND EXPRESSION OF CILIARY NEUROTROPHIC FACTOR (CNTF) [J].
LIN, LFH ;
MISMER, D ;
LILE, JD ;
ARMES, LG ;
BUTLER, ET ;
VANNICE, JL ;
COLLINS, F .
SCIENCE, 1989, 246 (4933) :1023-1025