EMBRYOGENESIS AND PLANT-REGENERATION FROM MICROSPORES OF BOTH INDICA AND JAPONICA RICE (ORYZA-SATIVA)

Microspore embryogenesis of rice (both indica and japonica types) was induced in culture yielding more than 200 green plants. Direct embryo germination and more frequently plant regeneration with multiple tillers have been obtained through secondary embryogenesis. Cold pre-treatment and the use of modified MS medium containing reduced nitrogen (glutamine and casein hydrolysate) and 10% (w/v) Ficoll might have favored microspore embryogenesis and green plant regeneration. Response of microspores in regeneration, embryogenesis and albinism varies depending on the constituents of the media and genotypes used. © 1990.