THE SURFACE-LOCATED YOPN PROTEIN IS INVOLVED IN CALCIUM SIGNAL TRANSDUCTION IN YERSINIA-PSEUDOTUBERCULOSIS

被引:216
作者
FORSBERG, A
VIITANEN, AM
SKURNIK, M
WOLFWATZ, H
机构
[1] UMEA UNIV,DEPT CELL & MOLEC BIOL,S-90187 UMEA,SWEDEN
[2] NATL DEF RES ESTAB,DEPT CELL & MICROBIOL,S-90182 UMEA,SWEDEN
[3] UNIV TURKU,DEPT MED MICROBIOL,SF-20520 TURKU 52,FINLAND
关键词
D O I
10.1111/j.1365-2958.1991.tb00773.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The low-calcium response (lcr) is strongly conserved among the pathogenic Yersinia species and is observed when the pathogen is grown at 37-degrees-C in Ca2+-depleted medium. This response is characterized by a general metabolic downshift and by a specific induction of virulence-plasmid-encoded yop genes. Regulation of yop expression is exerted at transcriptional level by a temperature-regulated activator and by Ca2+-regulated negative elements. The yopN gene was shown to encode a protein (formerly also designated Yop4b) which is surface-located when Yersinia is grown at 37-degrees-C. yopN was found to be part of an operon that is induced during the low-calcium response. Insertional inactivation of the yopN gene resulted in derepressed transcription of yop genes. A hybrid plasmid containing the yopN gene under the control of the tac promoter fully restored the wild-type phenotype of the yopN mutant. Thus the surface-located YopN somehow senses the calcium concentration and transmits a signal to shut off yop transcription when the calcium concentration is high.
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页码:977 / 986
页数:10
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