HETEROGENEITY WITHIN THE NONSTRUCTURAL PROTEIN-5-ENCODING REGION OF HEPATITIS-C VIRUSES FROM A SINGLE PATIENT

被引:29
作者
MURAKAWA, K
ESUMI, M
KATO, T
KAMBARA, H
SHIKATA, T
机构
[1] NIHON UNIV,SCH MED,DEPT PATHOL 1,OYAGUCHIKAMI MACHI,ITABASHI KU,TOKYO 173,JAPAN
[2] NIHON UNIV,SCH MED,MED RES INST,ITABASHI KU,TOKYO 173,JAPAN
[3] HITACHI LTD,CENT RES LAB,KOKUBUNJI,TOKYO 185,JAPAN
关键词
RNA VIRUS; MUTATION; QUASI-SPECIES; POLYMERASE CHAIN REACTION; CDNA CLONING; DNA SEQUENCING;
D O I
10.1016/0378-1119(92)90733-6
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Nucleotide (nt) sequence heterogeneity of the hepatitis C virus (HCV) genome derived from a single carrier was investigated. A polymerase chain reaction (PCR) product of 311 bp in the putative nonstructural protein 5-encoding region was directly sequenced, while part of a PCR product was cloned, and sequence analyses were carried out for 27 independent clones. Although 14 of the 27 clones were conserved, ten other types of nt sequences were found. The difference was at most 3 nt (1.1%). A directly determined sequence showed the major sequence of the cloned products. Since most of the nt changes occurred in the third letter of a codon, these nt changes might not have originated from random misincorporation during the PCR. These results of natural divergence of genome population in a single carrier suggest that HCV is a typical RNA virus with a quasi-species nature due to high mutation rates.
引用
收藏
页码:229 / 232
页数:4
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