IMPROVED INTRACELLULAR MORPHOLOGY OF PNEUMOCYSTIS-CARINII FROM RAT LUNG BY POSTFIXATION WITH A MIXTURE OF POTASSIUM FERROCYANIDE AND OSMIUM-TETROXIDE

被引:9
作者
GOHEEN, MP [1 ]
BLUMERSHINE, R [1 ]
BARTLETT, MS [1 ]
HULL, MT [1 ]
SMITH, JW [1 ]
机构
[1] RJ LEE GRP INC,MONROEVILLE,PA 15146
关键词
ULTRASTRUCTURE; TROPHOZOITES; CYSTS; PNEUMOCYSTIS-CARINII;
D O I
10.3109/10520299209110024
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Pneumocystis carinii infected rat lungs were postfixed with a mixture of OsO4 and K4Fe(CN)6. A marked improvement in staining of cell membranes, endoplasmic reticulum, nuclear membranes and glycogen was observed. These improvements were seen in both the trophic and cystic forms of the organisms. The addition of K4Fe(CN)6 did not improve the staining of cell walls, microtubules or ribosomes. Trophozoites were seen attached to both type 1 and type 2 pneumocytes by filopodia and/or intercalation of the cell body of P. carinii with the host lung cells. It is expected that the improvement in ultrastructural detail will allow better understanding of the ultrastructure of P. carinii and provide insights into the modes of action of various antimicrobial compounds on this organism.
引用
收藏
页码:140 / 148
页数:9
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