STEROID-PROTEIN INTERACTIONS .22. EXTRAPOLATION TECHNIQUE FOR DETERMINATION OF CORTICOSTEROID-BINDING GLOBULIN BY GEL FILTRATION

A method has been developed for the quantification of the corticosteroid-binding globulin (CBG) which eliminates the disadvantages of the gel filtration technique (dissociation, possible reversible adsorption). Binding capacity values are obtained with three different amounts of Sephadex G-50, and are extrapolated to a gel volume of zero. CBG concentrations have been measured for two normal and two pregnancy sera. The results have been found in agreement with those obtained by two independent procedures based on equilibrium dialysis. © 1969.