DIACETYLRHEIN AND RHEIN - INVIVO AND INVITRO EFFECT ON LYMPHOCYTE MEMBRANE FLUIDITY

被引:15
作者
BECCERICA, E
FERRETTI, G
CURATOLA, G
CERVINI, C
机构
[1] UNIV ANCONA,FAC MED & CHIRURG,IST BIOCHIM,VIA RANIERI,I-60131 ANCONA,ITALY
[2] UNIV ANCONA,FAC MED,RHEUMATOL UNIT,I-60100 ANCONA,ITALY
关键词
diacetylrhein; fluorescence polarization; lymphocyte membrane fluidity; osteoarthritis; rhein;
D O I
10.1016/1043-6618(90)90725-S
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The effect of diacetylrhein on lymphocyte membrane fluidity in osteoarthritis patients before and after 10 and 30 days of treatment was studied using the 1,6-diphenyl-1,3,5-hexatriene fluorescence polarization. Moreover we studied thein vitro effect of rhein, the active metabolite of diacetylrhein, on lymphocyte membrane fluidity of controls using the fluorescence polarization of 1,6-diphenyl1,3,5-hexatriene and its cationic derivative 1-(4-trimethylaminophenyl)-6-phenyl1,3,5-hexatriene. Our results showed that the patients with active osteoarthritis have higher fluorescence polarization values than those in the other osteoarthritis patients and in the controls. Moreover after 10 days of diacetylrhein treatment, we observed a significant decrease of fluorescence polarization values only in lymphocytes of the patients who showed active osteoarthritis before therapy. However after 30 days of treatment, significant changes of fluorescence polarization values were observed also in those patients who did not show painful osteoarthritis before diacetylrhein treatment. We observed thatin vitro rhein also induces an increase in lymphocyte membrane fluidity, more evident with 1-(4-trimethylaminophenyl)-6-phenyl-1,3,5-hexatriene with respect to 1,6-diphenyl1,3,5-hexatriene. © 1990 The Italian Pharmacological Society.
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页码:277 / 285
页数:9
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