QUANTITATION OF ULTRAVIOLET RADIATION-INDUCED CYCLOBUTYL PYRIMIDINE DIMERS IN DNA BY VIDEO AND PHOTOGRAPHIC DENSITOMETRY

被引：13

作者：

FREEMAN, SE

THOMPSON, BD

机构：

[1] Lovelace Medical Foundation, Albuquerque, NM 87108, 2425 Ridgecrest Drive, Southeast

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 186卷 / 02期

关键词：

Aromatic compounds - Electrophoresis - Ultraviolet radiation - Video cameras;

D O I：

10.1016/0003-2697(90)90070-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have compared video and photographic methods for calculating the number of ultraviolet radiation (uv)-induced pyrimidine dimers in DNA from the bacteriophage T7 exposed to uv (0 to 800 J/m2) from an FS40 sunlamp. DNA was incubated with a pyrimidine dimerspecific Micrococcus luteus uv endonuclease, subjected to alkaline agarose gel electrophoresis, neutralized, and stained with ethidium bromide, and the DNA fluorescence was recorded either with a video camera or on photographic film. The slopes of the dose-response curves for the number of uv-endonuclease-sensitive sites per 103 bases (pyrimidine dimers) was 1.2 (±0.1) × 10-4 uv-endonuclease-sensitive sites per J/m2 for the video analysis and 1.3 (±0.04) × 10-4 uv-endonuclease-sensitive sites per J/m2 for the photographic analysis. Results for pyrimidine dimer determination by either method were statistically comparable. © 1990.

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页码：222 / 228

页数：7

相关论文

共 22 条

[1] PHOTOREACTIVATION OF PYRIMIDINE DIMERS IN DNA FROM THYROID-CELLS OF THE TELEOST, POECILIA-FORMOSA [J].

ACHEY, PM ;

WOODHEAD, AD ;

SETLOW, RB .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1979, 29 (02) :305-310

[2] ENDONUCLEASE FROM MICROCOCCUS-LUTEUS WHICH HAS ACTIVITY TOWARD ULTRAVIOLET-IRRADIATED DEOXYRIBONUCLEIC ACID - PURIFICATION AND PROPERTIES [J].

CARRIER, WL ;

SETLOW, RB .

JOURNAL OF BACTERIOLOGY, 1970, 102 (01) :178-&

[3] EXCISION REPAIR OF UVR-INDUCED PYRIMIDINE DIMERS IN CORNEAL DNA [J].

FREEMAN, SE ;

APPLEGATE, LA ;

LEY, RD .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1988, 47 (01) :159-163

[4] EVALUATION OF DENSITOMETRY DATA USING INTERACTIVE COMPUTER-GRAPHICS - APPLICATION TO DNA AGAROSE GELS [J].

FREEMAN, SE ;

THOMPSON, BD .

INTERNATIONAL JOURNAL OF BIO-MEDICAL COMPUTING, 1988, 22 (02) :121-133

[5] VARIATIONS IN EXCISION REPAIR OF UVB-INDUCED PYRIMIDINE DIMERS IN DNA OF HUMAN-SKIN INSITU [J].

FREEMAN, SE .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1988, 90 (06) :814-817

[6] QUANTITATION OF RADIATION-INDUCED, CHEMICAL-INDUCED, OR ENZYME-INDUCED SINGLE-STRAND BREAKS IN NONRADIOACTIVE DNA BY ALKALINE GEL-ELECTROPHORESIS - APPLICATION TO PYRIMIDINE DIMERS [J].

FREEMAN, SE ;

BLACKETT, AD ;

MONTELEONE, DC ;

SETLOW, RB ;

SUTHERLAND, BM ;

SUTHERLAND, JC .

ANALYTICAL BIOCHEMISTRY, 1986, 158 (01) :119-129

[7] PRODUCTION OF PYRIMIDINE DIMERS IN DNA OF HUMAN-SKIN EXPOSED INSITU TO UVA RADIATION [J].

FREEMAN, SE ;

GANGE, RW ;

SUTHERLAND, JC ;

MATZINGER, EA ;

SUTHERLAND, BM .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1987, 88 (04) :430-433

[8] EXCISION REPAIR OF PYRIMIDINE DIMERS IN HUMAN PERIPHERAL-BLOOD LYMPHOCYTES - COMPARISON BETWEEN MITOGEN STIMULATED AND UNSTIMULATED CELLS [J].

FREEMAN, SE ;

RYAN, SL .

MUTATION RESEARCH, 1988, 194 (02) :143-150

[9] HIGHER PYRIMIDINE DIMER YIELDS IN SKIN OF NORMAL HUMANS WITH HIGHER UVB SENSITIVITY [J].

FREEMAN, SE ;

GANGE, RW ;

MATZINGER, EA ;

SUTHERLAND, BM .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1986, 86 (01) :34-36

[10] COMPARATIVE PROTECTION EFFICIENCY OF UVA-INDUCED AND UVB-INDUCED TANS AGAINST ERYTHEMA AND FORMATION OF ENDONUCLEASE-SENSITIVE SITES IN DNA BY UVB IN HUMAN-SKIN [J].

GANGE, RW ;

BLACKETT, AD ;

MATZINGER, EA ;

SUTHERLAND, BM ;

KOCHEVAR, IE .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1985, 85 (04) :362-364