CELL-SURFACE CHANGES IN THE PROTEINS OF RABBIT SPERMATOZOA DURING EPIDIDYMAL PASSAGE

被引：44

作者：

NICOLSON, GL ^{[1
]}

BRODGINSKI, AB ^{[1
]}

BEATTIE, G ^{[1
]}

YANAGIMACHI, R ^{[1
]}

机构：

[1] UNIV HAWAII,SCH MED,DEPT ANAT & REPROD BIOL,HONOLULU,HI 96822

来源：

GAMETE RESEARCH | 1979年 / 2卷 / 02期

关键词：

cell surface; epididymis; gel electrophoresis; membrane; proteins; spermatozoa; surface labeling;

D O I：

10.1002/mrd.1120020207

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Differences in the exposure of spermatozoa surface components during epididymal passage have been examined using lactoperoxidase‐catalyzed 125I‐iodination or labeling with 125I‐diazodiiodosulfanilic acid. Labeled surface proteins obtained from caput and cauda epididymides were solubilized in detergent, separated by sodium dodecylsulfate polyacrylamide slab gel electrophoresis, and identified by radiography. Densitometer scans of autoradiograms revealed increased amounts or exposures of surface proteins of ∼35,000, ∼39,000, ∼50,000, and ∼78,000 molecular weight on the cauda epididymal spermatozoa. Copyright © 1979 Alan R. Liss, Inc.

引用

页码：153 / 162

页数：10

共 44 条

[1]

BAKER LDS, 1970, J REPROD FERTIL, V22, P441

[2]

BEDFORD JM, 1965, J ANAT, V99, P891

[3]

BEDFORD JM, 1963, J REPROD FERTIL, V5, P169, DOI 10.1530/jrf.0.0050169

[4]

BEDFORD JM, 1971, J ANAT, V108, P527

[5] DEVELOPMENT OF FERTILIZING ABILITY OF SPERMATOZOA IN EPIDIDYMIS OF RABBIT [J].

BEDFORD, JM .

JOURNAL OF EXPERIMENTAL ZOOLOGY, 1966, 163 (03) :319-&

[6]

BEDFORD JM, 1978, MEMBRANE FUSION CELL, V5, P65

[7] MORPHOLOGY OF SPERMATOZOA FROM DIFFERENT LEVELS OF THE REPRODUCTIVE TRACT OF THE BULL [J].

BRANTON, C ;

SALISBURY, GW .

JOURNAL OF ANIMAL SCIENCE, 1947, 6 (02) :154-160

[8]

BROCHART M, 1953, CR SOC BIOL, V147, P20

[9]

CHULAVATNATOL M, 1976, J REPROD FERTIL, V48, P91, DOI 10.1530/jrf.0.0480091

[10]

EDWARDS RG, 1964, J REPROD FERTIL, V7, P153

← 1 2 3 4 5 →