A CONVENIENT HUMAN WHOLE-BLOOD CULTURE SYSTEM FOR STUDYING THE REGULATION OF TUMOR-NECROSIS-FACTOR RELEASE BY BACTERIAL LIPOPOLYSACCHARIDE

被引：96

作者：

WILSON, BMG ^{[1
]}

SEVERN, A ^{[1
]}

RAPSON, NT ^{[1
]}

CHANA, J ^{[1
]}

HOPKINS, P ^{[1
]}

机构：

[1] WELLCOME BIOTECH,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 139卷 / 02期

基金：

英国惠康基金;

关键词：

LIPOPOLYSACCHARIDE; ENDOTOXIN; EXVIVO; BLOOD; TUMOR NECROSIS FACTOR; CYTOKINE;

D O I：

10.1016/0022-1759(91)90193-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacterial lipopolysaccharide (LPS, endotoxin) induces a dose-dependent release of TNF in whole human blood which has been diluted five-fold. It is modulated by interferon-gamma, prostaglandin E2 and indomethacin in the same manner as observed with tumour necrosis factor (TNF) release from human monocyte/macrophage cells cultured in vitro. The whole blood culture system (WBCS) can provide up to 250 samples from 10 ml of venous blood and enables an individual blood to be assessed in terms of TNF inducibility and its modulation by other biological agents. The whole blood culture system was used to demonstrate the individual variation between blood donors. The results demonstrated that the information provided by induced cytokine release and its regulation in the ex vivo system would be a valuable addition to that obtained from in vitro methods.

引用

页码：233 / 240

页数：8

共 26 条

[1] THE COMMON MEDIATOR OF SHOCK, CACHEXIA, AND TUMOR NECROSIS [J].