LIVER-SPECIFIC INDUCTION OF NADPH-GENERATING ENZYMES BY POLYCHLORINATED-BIPHENYLS IN RATS

被引：12

作者：

HITOMI, Y ^{[1
]}

WAKAYAMA, M ^{[1
]}

ODA, H ^{[1
]}

YOSHIDA, A ^{[1
]}

机构：

[1] NAGOYA UNIV, DEPT AGR CHEM, CHIKUSA KU, NAGOYA 46401, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 07期

关键词：

D O I：

10.1271/bbb.57.1134

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The induction of NADPH-generating enzymes by polychlorinated biphenyls (PCB) in rats was investigated. The administration of PCB to rats for 3 and 14 days increased the activities of malic enzyme (ME, EC 1.1.1.40), glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49), and 6-phosphogluconate dehydrogenase (6PGD, EC 1.1.1.44) about 2-fold above the control level in the liver. Hepatic mRNA levels of ME, G6PD, and 6PGD, except for G6PD mRNA of the 14-day group, were also elevated to the same degree as the enzyme activities in PCB-treated rats. In rats fed a PCB-containing diet for 1 day, the hepatic mRNA levels of ME and G6PD were elevated prior to the induction of enzyme activity. In the kidney, lung, spleen, heart, and testis, the mRNA levels of ME, G6PD, and 6PGD were not affected by PCB. The induction of hepatic NADPH-generating enzymes would imply an increased demand of NADPH in the liver of rats fed with a PCB-containing diet.

引用

页码：1134 / 1136

页数：3

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