EFFECTS OF BUTHUS-MARTENSII (KARSCH) SCORPION-VENOM ON SODIUM CURRENTS IN RAT ANTERIOR-PITUITARY (GH3/B6) CELLS

被引：2

作者：

BAUER, CK ^{[1
]}

KRYLOV, B ^{[1
]}

ZHOU, PA ^{[1
]}

SCHWARZ, JR ^{[1
]}

机构：

[1] UNIV HAMBURG, KRANKENHAUS EPPENDORF, INST PHYSIOL, MARTINISTR 52, W-2000 HAMBURG 20, GERMANY

来源：

TOXICON | 1992年 / 30卷 / 5-6期

关键词：

D O I：

10.1016/0041-0101(92)90852-V

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effects of two fractions (II, containing anti-insect toxins, and III, containing eight anti-mammal toxins) isolated from the venom of the Old World scorpion Buthus martensii (Karsch) on Na+ currents of rat anterior pituitary cells (GH3/B-6 cells) were investigated using the whole-cell configuration of the patch clamp technique. Fraction II induced a temporary, and fraction Ill a permanent increase of the Na+ current amplitude. Application of each of the venom fractions resulted in a flattening of the curve relating steady state Na+ inactivation to membrane potential. In addition, the two fractions had specific effects. Fraction II shifted the voltage dependence of Na+ current activation by -42mV, and the voltage dependence of Na+ inactivation by -25 mV in the absence of a conditioning depolarizing pre-pulse. Slowing of Na+ inactivation was most prominent at negative membrane potentials, resulting in a steady Na+ inward current at the holding potential of -80 mV. Fraction III induced a pronounced slowing of Na+ inactivation leading to an increase of peak Na+ currents and to incomplete steady state Na+ inactivation even at positive membrane potentials.

引用

页码：581 / 589

页数：9

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