EXPRESSION OF MULTIPLE INTEGRINS AND EXTRACELLULAR-MATRIX COMPONENTS BY C6 GLIOMA-CELLS

被引：19

作者：

MALEKHEDAYAT, S

ROME, LH

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT BIOL CHEM,33-257 CHS,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,SCH MED,MENTAL RETARDAT RES CTR,LOS ANGELES,CA 90024

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 1992年 / 31卷 / 03期

关键词：

ADHESION RECEPTORS; FIBRONECTIN; LAMININ;

D O I：

10.1002/jnr.490310309

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have investigated the expression of integrins in C6 glioma, a chemically-induced glial tumor cell line from rat brain. Immunochemical analysis revealed that C6 cells express sets of integrin receptor complexes which immunologically and electrophoretically are indistinguishable from those expressed by normal rat skin fibroblasts. These include the well-characterized fibronectin (alpha-5-beta-1) and the multi-specific laminin, collagen and fibronectin (alpha-3-beta-1) receptors. Assay of cell adhesion indicated that C6 cells adhere to fibronectin-coated surfaces or matrix deposited by the C6 glioma cells (CGM) in an RGD- and divalent cation-dependent fashion. However, anti-fibronectin antibodies, which are able to inhibit fibroblast adhesion to fibronectin, did not inhibit adhesion of the C6 cells to fibronectin or CGM. This may reflect differences in functional properties and/or distribution patterns of integrins in C6 cells and normal fibroblasts.

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页码：470 / 478

页数：9

相关论文

共 34 条

[1] INTEGRINS AND OTHER CELL-ADHESION MOLECULES [J].

ALBELDA, SM ;

BUCK, CA .

FASEB JOURNAL, 1990, 4 (11) :2868-2880

[2] DIFFERENTIATED RAT GLIAL CELL STRAIN IN TISSUE CULTURE [J].

BENDA, P ;

LIGHTBODY, J ;

SATO, G ;

LEVINE, L ;

SWEET, W .

SCIENCE, 1968, 161 (3839) :370-+

[3] FOCAL ADHESIONS - TRANSMEMBRANE JUNCTIONS BETWEEN THE EXTRACELLULAR-MATRIX AND THE CYTOSKELETON [J].

BURRIDGE, K ;

FATH, K ;

KELLY, T ;

NUCKOLLS, G ;

TURNER, C .

ANNUAL REVIEW OF CELL BIOLOGY, 1988, 4 :487-525

[4] RGD-CONTAINING PEPTIDES INHIBIT THE SYNTHESIS OF MYELIN-LIKE MEMBRANE BY CULTURED OLIGODENDROCYTES [J].

CARDWELL, MC ;

ROME, LH .

JOURNAL OF CELL BIOLOGY, 1988, 107 (04) :1551-1559

[5] EVIDENCE THAT AN RGD-DEPENDENT RECEPTOR MEDIATES THE BINDING OF OLIGODENDROCYTES TO A NOVEL LIGAND IN A GLIAL-DERIVED MATRIX [J].

CARDWELL, MC ;

ROME, LH .

JOURNAL OF CELL BIOLOGY, 1988, 107 (04) :1541-1549

[6] REGULATION OF FIBRONECTIN RECEPTOR DISTRIBUTION BY TRANSFORMATION, EXOGENOUS FIBRONECTIN, AND SYNTHETIC PEPTIDES [J].

CHEN, WT ;

WANG, J ;

HASEGAWA, T ;

YAMADA, SS ;

YAMADA, KM .

JOURNAL OF CELL BIOLOGY, 1986, 103 (05) :1649-1661

[7] A NOVEL VITRONECTIN RECEPTOR INTEGRIN (ALPHA-V-BETA-X) IS RESPONSIBLE FOR DISTINCT ADHESIVE PROPERTIES OF CARCINOMA-CELLS [J].

CHERESH, DA ;

SMITH, JW ;

COOPER, HM ;

QUARANTA, V .

CELL, 1989, 57 (01) :59-69

[8] ISOLATION OF A NOVEL INTEGRIN RECEPTOR MEDIATING ARG-GLY-ASP DIRECTED CELL-ADHESION TO FIBRONECTIN AND TYPE-I COLLAGEN FROM HUMAN NEUROBLASTOMA-CELLS - ASSOCIATION OF A NOVEL BETA-1-RELATED SUBUNIT WITH ALPHA-V [J].

DEDHAR, S ;

GRAY, V .

JOURNAL OF CELL BIOLOGY, 1990, 110 (06) :2185-2193

[9] FIBRONECTIN RECEPTOR EXHIBITS HIGH LATERAL MOBILITY IN EMBRYONIC LOCOMOTING CELLS BUT IS IMMOBILE IN FOCAL CONTACTS AND FIBRILLAR STREAKS IN STATIONARY CELLS [J].

DUBAND, JL ;

NUCKOLLS, GH ;

ISHIHARA, A ;

HASEGAWA, T ;

YAMADA, KM ;

THIERY, JP ;

JACOBSON, K .

JOURNAL OF CELL BIOLOGY, 1988, 107 (04) :1385-1396

[10] ELEVATED LEVELS OF THE ALPHA-5-BETA-1-FIBRONECTIN RECEPTOR SUPPRESS THE TRANSFORMED PHENOTYPE OF CHINESE HAMSTER OVARY CELLS [J].

GIANCOTTI, FG ;

RUOSLAHTI, E .

CELL, 1990, 60 (05) :849-859

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