SELECTIVE HYDROLYSIS OF TRIGLYCERIDES WITH SURFACTANT-COATED LIPASE

Several surfactant - coated enzymes were prepared by coating various origin of lipase with nonionic surfactant dioleylglutamic acid ester ribtol (2C18 DELTA9GE) whose structure is similar to that of lipid. Selective hydrolysis reaction of triglycerides was investigated with the surfactant - coated lipase in organic media under water content - limited conditions. In this reaction system, the most suitable solvent is iso octane and the surfactant - coated lipase prepared by Candida cylindracea gives the highest hydrolysis yield of triolein. Selective hydrolysis of triolein can be performed by adjusting water content in iso octane. Assuming the Michaelis-Menten mechanism, apparent reaction rate constants were obtained by the analysis of initial reaction rate. The rate constant of surfactant - coated lipase was tens times that of powder lipase.