TYPING LISTERIA-MONOCYTOGENES - A COMPARISON OF RANDOM AMPLIFICATION OF POLYMORPHIC DNA WITH 5 OTHER METHODS

被引：50

作者：

BOERLIN, P

BANNERMAN, E

ISCHER, F

ROCOURT, J

BILLE, J

机构：

[1] CHU VAUDOIS,INST MICROBIOL,SWISS NATL CTR LISTERIOSIS,CH-1011 LAUSANNE,SWITZERLAND

[2] INST PASTEUR,CTR NATL REFERENCE LISTERIA,F-75724 PARIS 15,FRANCE

来源：

RESEARCH IN MICROBIOLOGY | 1995年 / 146卷 / 01期

关键词：

RAPD; LISTERIA MONOCYTOGENES; TYPING; EPIDEMIOLOGY; COMPARISON;

D O I：

10.1016/0923-2508(96)80269-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

One hundred Listeria monocytogenes strains were typed by random amplification of polymorphic DNA (RAPD) with three different primers, and the results were compared with those obtained by serotyping, ribotyping, multilocus enzyme electrophoresis, restriction enzyme analysis and phage typing. The RAPD patterns of strains appear to be stable during epidemics even over periods of several years. Reproducibility of the RAPD patterns was good. The discriminatory power of RAPD typing was the best among all the methods tested. RAPD is therefore a very promising tool in the study of listeriosis epidemiology. However, the problems related to the standardization of the technique first have to be resolved before the wide use of RAPD is possible.

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页码：35 / 49

页数：15

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