SPECIFICITY OF AN ESTERASE (XYLD) FROM PSEUDOMONAS-FLUORESCENS SUBSP CELLULOSA

被引：34

作者：

FAULDS, CB

RALET, MC

WILLIAMSON, G

HAZLEWOOD, GP

GILBERT, HJ

机构：

[1] INRA, BIOCHIM & TECHNOL GLUCIDES LAB, F-44026 NANTES, FRANCE

[2] BABRAHAM INST, DEPT CELLULAR PHYSIOL, CAMBRIDGE CB2 4AT, ENGLAND

[3] UNIV NEWCASTLE UPON TYNE, DEPT BIOL & NUTR SCI, NEWCASTLE UPON TYNE NE1 7RU, TYNE & WEAR, ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1995年 / 1243卷 / 02期

关键词：

ESTERASE; FERULIC ACID; OLIGOSACCHARIDE; (PSEUDOMONAS);

D O I：

10.1016/0304-4165(94)00140-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Activity of an esterase from Pseudomonas fluorescens subsp, cellulosa (XYLD) on an insoluble feruloylated hemicellulose substrate (de-starched wheat bran) was dependent on the source of added endo-xylanase. The esterase exhibited high selectivity for the nature, position of linkage and size of the feruloylated oligosaccharides generated by hydrolysis of the hemicellulose. Increased affinity of XYLD with increasing size of the oligosaccharide substrate suggests that optimal activity is observed on substrates with at least 4 sugars.

引用

收藏

页码：265 / 269

页数：5

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