A NEW RAPID ELECTROPHORETIC METHOD FOR DETERMINATION OF DEOXYRIBONUCLEIC ACID BASE COMPOSITION

被引：7

作者：

BORKOWSK.T

WOJCIERO.J

KULESZA, S

机构：

[1] Department of Physiological Chemistry, Medical School, Lublin

[2] Department of Human Genetics, Medical School, Lublin

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 27卷 / 01期

关键词：

D O I：

10.1016/0003-2697(69)90218-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is presented for the rapid analysis of DNA base composition. After separation from RNA by alkaline hydrolysis a DNA preparation was hydrolyzed with 72% HClO4 for 1 hr. After hydrolysis, HClO4 was removed by evaporation under reduced pressure. Dry powder containing the bases was dissolved in water and separated by agar gel electrophoresis. The spots of each base were cut out with agar gel and after dissolution in warm water were determined spectrophotometrically. The method may be applied to determination of base composition in 50 μg of analyzed DNA. © 1968.

引用

页码：58 / &

共 14 条

[1] METHODS FOR CHARACTERIZATION OF NUCLEIC ACIDS BY BASE COMPOSITION [J].