FRACTIONATION AND PRELIMINARY CHARACTERIZATION OF A LOW-MOLECULAR WEIGHT BOVINE HEPATIC INHIBITOR OF DNA-SYNTHESIS IN REGENERATING RAT-LIVER

被引：27

作者：

SEKAS, G ^{[1
]}

OWEN, WG ^{[1
]}

COOK, RT ^{[1
]}

机构：

[1] UNIV IOWA,COLL MED,DEPT PATHOL,IOWA CITY,IA 52242

来源：

EXPERIMENTAL CELL RESEARCH | 1979年 / 122卷 / 01期

关键词：

D O I：

10.1016/0014-4827(79)90559-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A heterogenous low molecular weight inhibitor of DNA synthesis in regenerating rat liver has been further fractionated by silica gel chromatography. The material is shown to contain a subfraction which stimulates isotope uptake in regenerating rat liver in vivo, as well as a strongly inhibitory subfraction which inhibits DNA synthesis up to 95%. The inhibitor fraction is effective both in vivo and in isolated rat liver nuclei from regenerating rat liver, whereas the stimulatory fraction is effective only in vivo. Other measurements of interest indicate that the unfractionated inhibitor preparation is not hepatotoxic, nor does it depress isotope uptake by the liver. These attributes are consistent with, but do not prove, the presence of a hepatic chalone. © 1979.

引用

页码：47 / 54

页数：8

共 28 条

[1] TENTATIVE MECHANISM OF LYMPHOCYTE CHALONE ACTION [J].