TIME COURSE AND PATTERN OF CORTICAL GRANULE BREAKDOWN IN HAMSTER EGGS AFTER SPERM FUSION

被引：26

作者：

STEWARTSAVAGE, J ^{[1
]}

BAVISTER, BD ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT VET SCI,MADISON,WI 53706

来源：

MOLECULAR REPRODUCTION AND DEVELOPMENT | 1991年 / 30卷 / 04期

关键词：

POLYSPERMY BLOCKS; TIMING; CORTICAL REACTION;

D O I：

10.1002/mrd.1080300414

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have determined the temporal relationship between sperm fusion and cortical granule breakdown in the hamster egg. Sperm fusion was determined by the Hoechst-transfer method (Stewart-Savage and Bavister: Dev Biol 128:150-157, 1988), and cortical granules were visualized with fluorescein isothiocynate-conjugated Lens culinaris agglutinin (Cherr et al. J Exp Zool 246:81-93, 1988). By 55 min after insemination, there was an 85% reduction in the density of cortical granules (fewer than four granules/100-mu-m2). Taking this value as the completion of the cortical reaction, analysis of the data indicate that the cortical reaction was completed 9 min after sperm fusion and 3 min after the formation of the zona and cell surface blocks to polyspermy. There was no obvious spatial pattern of granule loss in eggs that had a Hoechst-positive sperm but had not completed the cortical reaction.

引用

页码：390 / 395

页数：6

共 30 条

[1] CORTICAL GRANULES IN HAMSTER EGGS [J].