CIRCADIAN VARIATIONS OF ATRIAL-NATRIURETIC-PEPTIDE IN NORMAL PEOPLE AND ITS RELATIONSHIP TO ARTERIAL BLOOD-PRESSURE, PLASMA-RENIN ACTIVITY AND ALDOSTERONE LEVEL

To investigate the circadian variations of plasma atrial natriuretic peptide (ANP) and its relationship to arterial blood pressure, plasma renin activity and aldosterone level, we determined 24-h blood pressure in 14 healthy volunteers. Plasma ANP concentration, renin activity and aldosterone levels were measured every 3 h by radioimmunoassay. We found no significant circadian variation of plasma ANP level (pg/ml) (daytime level, 62 +/- 24 vs. night-time level, 57 +/- 19, P = 0.146) and plasma renin level (ng/ml/h) (1.32 +/- 0.78 vs. 1.15 +/- 0.57, P = 0.148), but there was diurnal change of blood pressure (mmHg) (systolic, 122 +/- 7 vs. 116 +/- 11, P < 0.001; diastolic, 80 +/- 11 vs. 72 +/- 11, P = 0.025) and plasma aldosterone level (pg/ml) (86 +/- 42 vs. 62 +/- 37, P < 0.001). The blood pressure and aldosterone levels reached maxima (11:00 h and 08:00 h, respectively) before that of ANP (17:00 h) and then decreased together until the nadir at 02:00 h. This might indicate that elevation of arterial blood pressure and plasma aldosterone level stimulate release of ANP under normal physiological conditions.