NUCLEOTIDE-SEQUENCE OF A UGA SUPPRESSOR SERINE TRANSFER-RNA FROM SCHIZOSACCHAROMYCES-POMBE

The UGA suppressor tRNA produced by Schizosaccharomyces pombe strain sup3-e was purified to homogeneity. It can be aminoacylated with serine by a crude aminoacyl-tRNA synthetase preparation from S. pombe cells. By combining post-labeling fingerprinting and gel sequencing methods the nucleotide sequence of this tRNA was determined to be: pG-U-C-A-C-U-A-U-G-U-C-ac4C-G-A-G-D-G-G-D-D-A-A-G-G-A-m2G-ψ-U-A-G-A-N-U-U*-C-A-i6A-A-ψ-C-U-A-A-U-G-G-G-C-U-U-U-G-C-C-C-G-m5C-G-C-A-G-G-T-ψ-C-A-m1A-A-U-C-C-U-G-C-U-G-G-U-G-A-C-G-C-C-AOH. The anticodon sequence U*CA is complementary to the UGA codon. © 1979 Information Retrieval Limited.