IMMUNOHISTOCHEMICAL EVIDENCE FOR AN ASSOCIATION OF RIBOSOMES WITH MICROFILAMENTS IN 3T3 FIBROBLASTS

被引：22

作者：

HESKETH, JE

HORNE, Z

CAMPBELL, GP

机构：

[1] Biochemical Sciences Division, Rowett Research Institute, Aberdeen, AB2 9SB, Greenburn Rd., Bucksburn

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1991年 / 15卷 / 02期

关键词：

D O I：

10.1016/0309-1651(91)90105-R

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Ribosome distribution in cultured fibroblasts was investigated immunohistochemically using antibodies which recognize the 60S ribosomal subunit. After treatment of cells with buffer containing 25mM KCl and 0.05% Nonidet-P40 immunostained material was present in punctate patterns and linear arrays consistent with some ribosomes being associated with the cytoskeleton. Treatment of the cells with 130mM KCl caused loss of both the beaded lines of immunostaining and micro-filaments. Double immunostaining showed ribosomes to be closely associated with microfilaments. © 1991.

引用

收藏

页码：141 / 150

页数：10

相关论文

共 20 条

[1]

AGUTTER PS, 1988, PROGR MOL SUBCELLULA, V10, P15

[2] FRACTIONATION OF MEMBRANE-BOUND POLYSOMES, FREE POLYSOMES, AND NUCLEI FROM TISSUE-CULTURED CELLS [J].

BIRCKBICHLER, PJ ;

PRYME, IF .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1973, 33 (02) :368-373

[3] CYTOSKELETON INVOLVEMENT IN THE DISTRIBUTION OF MRNP COMPLEXES AND SMALL CYTOPLASMIC RNAS [J].

BIRD, RC ;

SELLS, BH .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 868 (04) :215-225

[4] TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA [J].

BLOBEL, G ;

DOBBERSTEIN, B .

JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :835-851

[5] THE SPATIAL-DISTRIBUTION OF POLYRIBOSOMES IN 3T3-CELLS AND THE ASSOCIATED ASSEMBLY OF PROTEINS INTO THE SKELETAL FRAMEWORK [J].

FULTON, AB ;

WAN, KM ;

PENMAN, S .

CELL, 1980, 20 (03) :849-857

[6] EVIDENCE THAT INSULIN INCREASES THE PROPORTION OF POLYSOMES THAT ARE BOUND TO THE CYTOSKELETON IN 3T3 FIBROBLASTS [J].

HESKETH, JE ;

PRYME, IF .

FEBS LETTERS, 1988, 231 (01) :62-66

[7] ASSOCIATION OF MESSENGER-RNA AND ELF-2-ALPHA WITH THE CYTOSKELETON IN CELLS LACKING VIMENTIN [J].

HEUIJERJANS, JH ;

PIEPER, FR ;

RAMAEKERS, FCS ;

TIMMERMANS, LJM ;

KUIJPERS, H ;

BLOEMENDAL, H ;

VANVENROOIJ, WJ .

EXPERIMENTAL CELL RESEARCH, 1989, 181 (02) :317-330

[8] IMMUNOLOGICAL LOCALIZATION OF RIBOSOMES IN STRIATED RAT MUSCLE - EVIDENCE FOR MYOFIBRILLAR ASSOCIATION AND ONTOLOGICAL CHANGES IN THE SUBSARCOLEMMAL MYOFIBRILLAR DISTRIBUTION [J].

HORNE, Z ;

HESKETH, J .

BIOCHEMICAL JOURNAL, 1990, 268 (01) :231-236

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10]

LASTICK SM, 1976, J BIOL CHEM, V251, P2867