INTRODUCTION OF THE TAC-PROMOTER BY LACTOSE UNDER FERMENTATION CONDITIONS

被引：18

作者：

NEUBAUER, P ^{[1
]}

WOLFF, C ^{[1
]}

HECKER, M ^{[1
]}

HOFMANN, K ^{[1
]}

MEYER, L ^{[1
]}

KRUSCHKE, P ^{[1
]}

HEINRICH, HW ^{[1
]}

机构：

[1] FRIEDRICH LOFFLER INST INSEL RIEMS,MOLEK BIOL ABT,W-2201 INSEL RIEMS,GERMANY

来源：

ACTA BIOTECHNOLOGICA | 1991年 / 11卷 / 01期

关键词：

D O I：

10.1002/abio.370110107

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The use of the expensive IPTG for induction of the tac-promoter is not practicable at the industrial scale because of the large amounts necessary for induction. We developed a system in which IPTG as inducer was replaced by lactose which induces the tac-promoter with the same efficiency. In E. coli CW3011 (pOF1.0) lactose is utilized as inducer and carbon source even in the presence of glucose, presumably as a consequence of the overproduction of lac-permease.

引用

页码：23 / 29

页数：7

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