ELIMINATION OF ADSORPTION EFFECTS IN GEL-PERMEATION CHROMATOGRAPHY OF HUMIC SUBSTANCES

被引：50

作者：

TOWN, RM

POWELL, HKJ

机构：

[1] Department of Chemistry, University of Canterbury, Private Bag

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 256卷 / 01期

关键词：

GEL PERMEATION CHROMATOGRAPHY; ADSORPTION; HUMIC SUBSTANCES; MOLECULAR SIZE;

D O I：

10.1016/0003-2670(92)85330-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Elution of humic substances from Sephadex G-100 gel was optimized by use of samples filtered through a 0.025-mu-m membrane filter and 0.01 mol l-1 disodium tetraborate eluent. However, some material was still retained on the column, probably through complexing to metal ions bound to the gel. This problem was eliminated by addition of 0.001 mol l-1 Na4P2O7 to the eluent.

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页码：81 / 86

页数：6

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