FINE-STRUCTURE OF THE DORSAL CAP OF THE INFERIOR OLIVE AND ITS GABAERGIC AND NONGABAERGIC INPUT FROM THE NUCLEUS PREPOSITUS-HYPOGLOSSI IN RAT AND RABBIT

The dorsal cap of the inferior olive is involved in the control of eye movements and is excited by inputs from the midbrain. In the present study we attempted to determine the inhibitory input to this nucleus in rat and rabbit. The projection from the nucleus prepositus hypoglossi to the dorsal cap was studied in the light microscope by anterograde tracing of Phaseolus vulgaris-leucoagglutinin and lesion-induced depletion of glutamic acid decarboxylase immunoreactivity, and in the electron microscope by anterograde tracing of wheat germ agglutinin-coupled horseradish peroxidase combined with GABA immunocytochemistry. We show that the nucleus prepositus hypoglossi projects bilaterally to the dorsal cap, contralaterally to the ventrolateral outgrowth, and ipsilaterally to the medial accessory olive. After lesioning of the nucleus prepositus hypoglossi, the caudal dorsal cap was depleted of most of its glutamic acid decarboxylase-immunoreactive terminals while the rostral dorsal cap and the ventrolateral outgrowth were depleted of a minor part. Ultrastructural analysis indicates that the majority, but not all, of the terminals from the nucleus prepositus hypoglossi in the dorsal cap are GABA-positive. These GABA-positive and GABA-negative terminals form predominantly symmetric and asymmetric synapses; most of them synapse on dendrites outside and inside glomeruli, frequently in association with dendrodendritic gap junctions, while a small minority are axosomatic. None of the terminals from the nucleus prepositus hypoglossi was found to form a crest synapse, although synapses of this kind were predominantly formed by GABAergic terminals. This study shows that the dorsal cap receives a major inhibitory input from the nucleus prepositus hypoglossi, the terminals of which are located at strategic positions on the olivary neurons.