ECM GENE-EXPRESSION AND ITS MODULATION BY INSULIN IN DIABETIC RATS

被引：162

作者：

FUKUI, M ^{[1
]}

NAKAMURA, T ^{[1
]}

EBIHARA, I ^{[1
]}

SHIRATO, I ^{[1
]}

TOMINO, Y ^{[1
]}

KOIDE, H ^{[1
]}

机构：

[1] JUNTENDO UNIV,SCH MED,DEPT MED,DIV NEPHROL,TOKYO 113,JAPAN

来源：

DIABETES | 1992年 / 41卷 / 12期

关键词：

D O I：

10.2337/diabetes.41.12.1520

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The steady-state levels of mRNA encoding for the alpha1(IV) collagen chain, laminin B1 and B2 chains, basement membrane HSPG, and alpha1(I) and alpha1(III) collagen chains were examined in rat glomeruli at 4, 12, and 24 wk after injection of STZ. The mRNA levels for the alpha1(IV) collagen chain, laminin B1 and B2 chains, and alpha1(I) and alpha1(III) collagen chains increased significantly with age in the STZ-induced diabetic rats before morphological thickening of basement membrane occurred. In contrast, the mRNA levels for HSPG decreased markedly 4 wk after STZ injection and then increased with age compared with those for control rats. The mRNA levels for these ECM components showed a continuous decline with age in controls. Treating the diabetic rats with insulin for 4 wk ameliorated the abnormally regulated ECM gene expression in the glomeruli. These data suggest that the abnormal regulation of ECM gene expression in the glomeruli may contribute to the expansion of mesangial matrix and basement membrane thickening in diabetic rats, and that hyperglycemia may play a role in the abnormal ECM gene expression.

引用

页码：1520 / 1527

页数：8

共 47 条

[1] INCREASED EXTRACELLULAR-MATRIX SYNTHESIS AND MESSENGER-RNA IN MESANGIAL CELLS GROWN IN HIGH-GLUCOSE MEDIUM [J].

AYO, SH ;

RADNIK, RA ;

GLASS, WF ;

GARONI, JA ;

RAMPT, ER ;

APPLING, DR ;

KREISBERG, JI .

AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (02) :F185-F191

[2]

AYO SH, 1990, AM J PATHOL, V136, P1339

[3] S-35-LABELED GLYCOSAMINOGLYCAN AND S-35-LABELED GLYCOPEPTIDE METABOLISM BY DIABETIC GLOMERULI AND AORTA [J].

BROWN, DM ;

KLEIN, DJ ;

MICHAEL, AF ;

OEGEMA, TR .

DIABETES, 1982, 31 (05) :418-425

[4] CHARACTERISTICS AND MECHANISMS OF HIGH-GLUCOSE INDUCED OVEREXPRESSION OF BASEMENT-MEMBRANE COMPONENTS IN CULTURED HUMAN ENDOTHELIAL-CELLS [J].

CAGLIERO, E ;

ROTH, T ;

ROY, S ;

LORENZI, M .

DIABETES, 1991, 40 (01) :102-110

[5] INCREASED EXPRESSION OF BASEMENT-MEMBRANE COMPONENTS IN HUMAN-ENDOTHELIAL CELLS CULTURED IN HIGH GLUCOSE [J].

CAGLIERO, E ;

MAIELLO, M ;

BOERI, D ;

ROY, S ;

LORENZI, M .

JOURNAL OF CLINICAL INVESTIGATION, 1988, 82 (02) :735-738

[6] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[7] GENOMIC SEQUENCING [J].

CHURCH, GM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995

[8] NUMBER AND EVOLUTIONARY CONSERVATION OF ALPHA-TUBULIN AND BETA-TUBULIN AND CYTOPLASMIC BETA-ACTIN AND GAMMA-ACTIN GENES USING SPECIFIC CLONED CDNA PROBES [J].

CLEVELAND, DW ;

LOPATA, MA ;

MACDONALD, RJ ;

COWAN, NJ ;

RUTTER, WJ ;

KIRSCHNER, MW .

CELL, 1980, 20 (01) :95-105

[9]

COHEN MP, 1981, J LAB CLIN MED, V98, P715

[10] RENAL GLOMERULAR COLLAGEN-SYNTHESIS IN STREPTOZOTOCIN DIABETES - REVERSAL OF INCREASED BASEMENT-MEMBRANE SYNTHESIS WITH INSULIN THERAPY [J].

COHEN, MP ;

KHALIFA, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 500 (02) :395-404

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