SUPEROXIDE RADICALS IN INTACT TISSUES AND IN DIMETHYL SULFOXIDE-BASED EXTRACTS

被引：15

作者：

JACKS, TJ

HINOJOSA, O

机构：

[1] Southern Regional Research Center, U.S. Department of Agriculture, New Orleans, LA 70179

来源：

PHYTOCHEMISTRY | 1993年 / 33卷 / 03期

关键词：

CYPERUS-ROTUNDUS; CYPERACEAE; GLYCINE MAX; LEGUMINOSEAE; GOSSYPIUM-HIRSUTUM; MALVACEAE; LIGUSTRUM-VULGARE; OLEACEAE; PINUS-ECHINATA; PINACEAE; ZEA-MAYS; GRAMINEAE; ELECTRON PARAMAGNETIC RESONANCE; FREE RADICALS; SUPEROXIDE; ASCORBATE;

D O I：

10.1016/0031-9422(93)85449-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Steady state contents of free radicals were investigated in intact living tissues and in tissue extracts prepared with dimethyl sulphoxide-tetrabutylammonium perchlorate (DMSO-TBAP). DMSO-TBAP was suitable for extraction because free radical artifacts were not generated and hydrous superoxide was both stable and readily spin-trapped for electron paramagnetic resonance (EPR) spectrometry. Intact leaves and roots contained an unresolved radical and paramagnetic manganese. Superoxide was EPR-silent in whole tissues but was spin-trapped in extracts of roots where its steady-state concentration in vivo was approximately 10(-5) M. Ascorbyl radicals were generated upon alkalization of whole tissues and by comminution of tissues in DMSO-TBAP. Aprotic sites for the storage of superoxide in vivo are considered and a role for storage is discussed in regards to cytochemical defence mechanisms of plant protection.

引用

页码：563 / 568

页数：6

共 43 条

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Afanas'ev I.B., 1989, SUPEROXIDE ION CHEM

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