DETERMINATION BY NMR-SPECTROSCOPY OF THE STRUCTURE AND CONFORMATIONAL FEATURES OF THE ENTEROBACTERIAL COMMON ANTIGEN ISOLATED FROM ESCHERICHIA-COLI

被引：16

作者：

BRUIX, M

JIMENEZBARBERO, J

CRONET, P

机构：

[1] CSIC, INST QUIM ORGAN, E-28006 MADRID, SPAIN

[2] EUROPEAN MOLEC BIOL LAB, HEIDELBERG, GERMANY

来源：

CARBOHYDRATE RESEARCH | 1995年 / 273卷 / 02期

关键词：

SUGAR CONFORMATION; ENTEROBACTERIAL COMMON ANTIGEN (EGA); ECA STRUCTURE; NMR OF ECA;

D O I：

10.1016/0008-6215(95)00117-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complete H-1 and C-13 spectrum of a polysaccharide isolated from Escherichia coli, which is the major component of the enterobacterial common antigen, has been analyzed through two-dimensional nuclear magnetic resonance spectroscopy. In addition, distance constraints from NOESY and ROESY experiments have been combined with molecular dynamic simulations to determine its major conformation in water solution. Data resulting from both free dynamic simulations and restrained dynamic simulations have been compared with experimental data and discussed.

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页码：157 / 170

页数：14

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