AGGREGATED DNA IN ETHANOL SOLUTION

A recently developed mechanochemical method has provided a new, efficient tool for studies on the thermal stability and structure of aggregated DNA in ethanol-water solutions, At low ethanol concentrations DNA is fully soluble and is in the B form, However, with increasing ethanol concentration the melting temperature of DNA, T-m, decreases. At a critical ethanol concentration, dependent on the nature and concentration of the counterion, aggregation of the DNA molecules sets in. This is reflected in a marked increase in T-m indicating that the aggregated DNA molecules are thermally more stable than the dissolved ones. However, they are still in the B form. In general, T-m of aggregated DNA also decreases with further increasing ethanol concentration and is dependent on the nature of the counterion, but T-m is not affected by the concentration of the counterion (excess salt) in the ethanol-water solution, When the ethanol concentration reaches the range of 70-80% (v/v), the B-to-A conformational transition occurs in the case of Na-, K- and CsDNA. Above this transition point the A form is more stable than the B form due to the reduced mater activity and to increased interhelical interactions. At very high ethanol concentrations, above 85% and dependent on the nature of the counterion, a drastic change in the thermal behaviour is observed, Apparently such a strong interhelical interaction is induced in the aggregated DNA that the DNA is stabilized and cannot adopt a random coil state even at very high temperatures, This stability of DNA in the P form is fully reversed if the ethanol concentration is lowered and the activity of water, thereby, is restored.