MODULATION OF GUANINE TRIPHOSPHATE NUCLEOTIDE BINDING TO P21(RAS) IMMUNOPRECIPITATES OF RAT-LIVER PLASMA-MEMBRANES BY AGENTS AFFECTING REDOX STATE

被引：4

作者：

WILKINSON, FE ^{[1
]}

PAULIK, M ^{[1
]}

MORRE, DJ ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT MED CHEM,W LAFAYETTE,IN 47907

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 190卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.1035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

GTP-γ-[35S] and GTP-γ-[32P] or GTP-α-[32P] bound to plasma membranes of rat liver was immunoprecipitated using anti p21(V-H-ras). Binding was enhanced approximately 2-fold by incubation with an exogenous electron acceptor, potassium ferricyanide (but not with potassium ferrocyanide), or oxidized ubiquinone10 and was inhibited or unaffected by incubation with reduced pyridine nucleotides (NADH or NADPH) or reduced ubiquinone10. The results suggest a mechanism of guanine nucleotide exchange that is responsive to oxidation-reduction. © 1993 Academic Press, Inc.

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页码：229 / 235

页数：7

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