ACTIVATION OF FNR-DEPENDENT TRANSCRIPTION BY IRON - AN IN-VITRO SWITCH FOR FNR

被引：11

作者：

GREEN, J ^{[1
]}

GUEST, JR ^{[1
]}

机构：

[1] UNIV SHEFFIELD,KREBS INST BIOMOLEC RES,DEPT MOLEC BIOL & BIOTECHNOL,WESTERN BANK,SHEFFIELD S10 2UH,ENGLAND

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 113卷 / 02期

关键词：

FNR; TRANSCRIPTIONAL REGULATION; IRON; TRANSCRIPTION; IN-VITRO; REDOX-SENSING; ESCHERICHIA-COLI;

D O I：

10.1016/0378-1097(93)90272-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

FNR is an iron-binding transcriptional regulator of Escherichia coli which controls the expression of target genes in response to anaerobiosis. The mechanism used by FNR to sense and respond to anaerobiosis is unknown but it is thought to involve iron. In vitro transcription analyses have shown that iron-deficient FNR is unable to activate transcription from the FF-melR promoter, but activity could be restored by preincubation with Fe2+ and beta-mercaptoethanol. The reactivation of FNR was prevented and reversed by chelating agents, and this reactivation thus provides an artificial in vitro switch for FNR-dependent transcriptional activation.

引用

页码：219 / 222

页数：4

共 16 条

[1] THE EFFECT OF IRON LIMITATION ON EXPRESSION OF THE AEROBIC AND ANAEROBIC ELECTRON-TRANSPORT PATHWAY GENES IN ESCHERICHIA-COLI [J].