ENZYMATIC DIGESTION OF DNA GAMMA-IRRADIATED IN AQUEOUS-SOLUTION SEPARATION OF DIGESTS BY ION-EXCHANGE CHROMATOGRAPHY

被引：23

作者：

DIZDAROGLU, M

HERMES, W

SCHULTEFROHLINDE, D

VONSONNTAG, C

机构：

[1] Institut für Strahlenchemie im Max-Planck-Institut für Kohlenforschung, D-4330, Mülheim a.d. Ruhr

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1978年 / 33卷 / 06期

关键词：

D O I：

10.1080/09553007814550471

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Aqueous solutions of DNA were γirradiated in the presence and absence of oxygen and enzymatically hydrolysed by the combined action of pancreatic deoxyribonuclease (DNase I), snake-venom phosphodiesterase (PDE I), spleen phosphodiesterase (PDE II) and alkaline phosphatase. In contrast to unirradiated DNA, which is fully hydrolysed to nucleosides by these enzymes, γirradiated DNA yields a series of oligonucleotides. Their isolation might enable the future identification of the chemical nature of DNA lesions. © 1978 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：563 / 569

页数：7

共 27 条

[1] [Anonymous], 1971, ENZYMES, V4, P313
[2] BEESK F, INT J RAD BIOL
[3] Bernardi A., 1971, ENZYMES, V4, P329
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[5] BOLLUM FJ, 1965, J BIOL CHEM, V240, P2599
[6] DAVIDSON JN, 1972, BIOCH NUCLEIC ACIDS
[7] DIZDAROGLU M, 1977, Z NATURFORSCH C, V32, P1021
[8] DIZDAROGLU M, 1976, Z NATURFORSCH B, V31, P227
[9] STRAND BREAKS AND SUGAR RELEASE BY GAMMA-IRRADIATION OF DNA IN AQUEOUS-SOLUTION
DIZDAROGLU, M
SONNTAG, CV
SCHULTEFROHLINDE, D
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1975, 97 (08) : 2277 - 2278
[10] ISOLATION OF 2-DEOXY-D-ERYTHRO-PENTONIC ACID FROM AN ALKALI-LABILE SITE IN GAMMA-IRRADIATED DNA
DIZDAROGLU, M
SCHULTEFROHLINDE, D
VONSONNTAG, C
[J]. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1977, 32 (05) : 481 - 483

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